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Unexpected lessons from conducting research with older adults

Julia Nolte: “Becoming a gerontologist has taught me many unexpected things about the way the “stages of life” translate into lifespan research. Given these surprising lessons, my advice to other budding researchers is this: know that being an expert in your field is not only about the formal knowledge you amass.”

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Julia Nolte

[su_boxbox title=”About”]Julia Nolte earned a B.Sc. in Psychology from Heidelberg University, Germany, in 2014 followed by an M.Sc. in Psychology in 2017. During her time in Heidelberg, Julia also completed research visits at Cornell University (2015 – 2016), Harvard University (2016), Oxford University (2016), and Cambridge University (2017). Since 2017, Julia has been an M.A./ Ph.D. student at Cornell University’s Human Development Department. In her doctoral work, Julia focuses on the intersection of decision making, lifespan development, and health. You can follow Julia on Twitter here: @Julia_Nolte92. Image by Gerd Altmann from Pixabay. [/su_boxbox]

[su_boxnote note_color=”#c8c8c8″]Story key points:

  • Being an expert isn’t only about formal knowledge – real expertise comes from the lessons you will learn beyond the classroom or your desk.
  • Don’t pursue your path blindly. Before you start, try to take stock of the things you don’t know.
  • If your research involves people, get to know them better – they are your most valuable teachers.[/su_boxnote]

Julia Nolte

[dropcap]A[/dropcap]t some point, you have probably come across this take on the “stages of life:” teenagers have time and energy, but no money; adults in the workforce have money and energy, but no time; and older adults have time and money, but no energy to enjoy them.

If you have encountered these “stages” before, I assume that you have never stopped to consider how different phases of life impact the way scientists conduct lifespan research. For a long time, I didn’t pay the “stages” any mind myself. Now I regret not recognizing their value sooner: If I had, I believe I could have avoided many mistakes I made in my own lifespan research.

Instead, I started my Ph.D. on the psychology of aging with little idea what I was getting myself into.

Prior to my doctoral work, I had no access to the resources necessary to conduct lab-based experiments involving different age groups. Instead, I carried out online studies with the help of strangers who never actually interacted with me. As a result, I had no hands-on experience working with older adults before I committed my life to studying them.

In the years that have passed since then, I have learned a thing or two about aging research that I never thought I would. Here are the lessons that struck me the most.

First, I need to provide some context. When I entered college as a psychology major, I had no intentions of pursuing research, let alone aging research. It wasn’t until I completed an internship on Alzheimer’s disease that I fell in love with lifespan development. My internship entailed answering emails about the prevention, diagnosis, and treatment of dementia that my research institution received from members of the public. To answer these questions, I had to screen and summarize the research record, which often led me to discovering knowledge that I wish everyone had access to. Suddenly, “research” and “aging” were no longer abstract constructs to me. Instead, I realized that studying aging was my best approximation of a super power: anticipating – and knowing how to improve – people’s futures, particularly with regard to their health.

Younger, middle-aged, and older adults do not share the same motivation to participate in research studies.

Although I now study decision making rather than Alzheimer’s disease, I have stayed true to wondering how we can help older adults live long and healthy lives. But to research how aging influences health-related decision making, I never study older adults alone. Instead, I typically compare adults over the age of 60 to younger adults under the age of 35 or to a whole lifespan sample that also includes middle-aged adults.

Much like the “stages of life” suggest, younger, middle-aged, and older adults do not share the same motivation to participate in research studies. Middle-aged adults are the hardest to recruit for in-person experiments. Because they work but also take care of children or older relatives, middle-aged adults are notoriously busy. In addition, the payment we can offer them is often not sizable enough to be worth their time. As a result, my lab-based research is generally limited to studying younger and older adults.

Fortunately, recruiting younger adults into a study is comparatively easy. Because many younger adults have not fully entered the workforce yet, they are drawn in by the promise of payment or extra course credit (Sharp, Pelletier, & Lévesque, 2006). While they often don’t have to care for any dependents, young adults are similar to middle-aged adults in one regard: Like middle-aged adults, younger adults often have places to go and things to do. As a consequence, younger adults will be swift when completing a study and rarely take the time to exchange pleasantries with us researchers.

Older adults, on the other hand, perceive studies as an opportunity to get out of the house and socialize (e.g., Baczynska, Shaw, Patel, Sayer, & Roberts, 2017). In my experience, many older participants enjoy sharing their life stories with researchers and like to strike up conversations during or after an experiment. Not knowing this, I once made the mistake of inviting several older adults to a group testing session. In this session, multiple participants completed a study at the same time. However, each participant was supposed to work quietly by themselves. Although this set-up works well when testing younger adults, bringing together older adults proved to be a real challenge. Rather than focusing on their own answers, older adults started discussing the study, laughed out loud when encountering unexpected questions, and commented on each other’s experiences, such as one man’s struggle to use a broken computer mouse. Needless to say, the group session did not provide the quiet testing environment I had needed to collect quality data. Since then, I have always opted for one-on-one sessions, especially when working with older adults.

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Unlike many younger adults, I find that some older adults are not “in it for the money” when participating in research (e.g., Friesen & Williams, 2016). In fact, several older adults have asked me whether we could set up a donation box for those participants who did not want to accept the payment we provide. Of course, it is true that older adults sometimes participate in studies in order to supplement their retirement income or savings. However, many older adults are more enticed to participate by their intellectual interest in research, by altruistic motivations, or the wish to learn about themselves (Baczynska et al., 2017; Jun, Hsieh, & Reinecke, 2017). These participants will often inquire about the goals of a study or contact us to provide feedback about their experiences. “You shouldn’t ask this question” or “this question could be misinterpreted” are sentences I hear often, but typically not from my younger participants.

Older adults keep surprising me with the strategies they use to cope with cognitive loss or other age-related limitations.

Likewise, many of my older participants are “research veterans,” meaning they have already participated in a lot of different research studies. As a result, they will often inform me that they don’t enjoy certain cognitive tests or that they will try hard to “beat” their previous best performance on a task. As researchers, we don’t always account for the fact that our participants might not be the blank slates we hope to encounter. This is problematic because previous experience with a test can sometimes distort our findings. Therefore, working with older adults has taught me to include questions about prior exposure to various types of tasks into my most recent studies.

Finally, I have found that a controlled lab environment is not a good reflection of older participants’ lived experiences. I know this because older adults keep surprising me with the strategies they use to cope with cognitive loss or other age-related limitations. Imagine my bewilderment when an older adult took out their phone to use as a calculator when asked to complete a mathematical task that I had hoped they would be solving in their heads. In a similar instance, I was dismayed to find that an older adult had used their copy of the consent form to take notes during a memory test. Although I champion these behaviors in older adults’ day-to-day lives, “cheating” on assessments invalidates the data I collect in my studies. Since gaining first-hand experience with older participants, I have become more vigilant during data collection and take extra caution to formulate clear task instructions.

In sum, becoming a gerontologist has taught me many unexpected things about the way the “stages of life” translate into lifespan research. Given these surprising lessons, my advice to other budding researchers is this: know that being an expert in your field is not only about the formal knowledge you amass. Instead, expertise also consists of the many small things you cannot learn in the classroom or from behind your desk. Take stock of the things you do know and the things you might not know about the research you aim to conduct. Rather than only looking for the science generated by your field, seek out handbooks, blog posts, best-practice writing, and other unspoken knowledge on how to actually complete research within your field. Ask your mentors and more experienced students about what they wish they had known before starting their careers or which skills they needed to acquire in order to succeed in those careers. Then pay it forward by sharing your insights with those who are less experienced than you.

But most importantly, if your research involves people, get to know them better. In many ways, they will be your most valuable teachers.

References:

Baczynska, A. M., Shaw, S. C., Patel, H. P., Sayer, A. A., & Roberts, H. C. (2017). Learning from older peoples’ reasons for participating in demanding, intensive epidemiological studies: A qualitative study. BMC Medical Research Methodology, 17, 167. doi: 10.1186/s12874-017-0439-9

Friesen, L. R., & Williams, K. B. (2016). Attitudes and motivations regarding willingness to participate in dental clinical trials. Contemporary Clinical Trials Communications, 2, 85-90. doi: 10.1016/j.conctc.2015.12.011

Jun, E., Hsieh, G., & Reinecke, K. (2017). Types of motivation affect study selection, attention, and dropouts in online experiments. Proc. ACM Hum.-Comput. Interact, 1(1), 1-15. https://doi.org/10.1145/3134691

Sharp, E. C., Pelletier, L. G., & Lévesque, C. (2006).  The double-edged sword of rewards for participation in psychology experiments. Canadian Journal of Behavioral Science, 38(3), 269-277. doi: 10.1037/cjbs2006014

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CivicSciTimes - Stories in Science

Unexpected Stories and Spindle Mistakes: Discovering that Wild-type Cells are Full of Surprises

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Natalie Nannas

Natalie Nannas is an Associate Professor of Biology at Hamilton College in Clinton, NY. She teaches courses in genetics, molecular biology, and bioethics. Dr. Nannas graduated from Grinnell College with bachelor’s degrees in biological chemistry and French. She received her Master’s and PhD from Harvard University in molecular biology and genetics. Dr. Nannas conducted her postdoctoral research at the University of Georgia where she won a National Science Foundation Plant Genome Postdoctoral Fellowship. At Hamilton College, Dr. Nannas enjoys teaching and sharing her passion for microscopy with her undergraduate research students. When not glued to a microscope, she loves spending time with her husband and two daughters. The narrative below by Natalie Nannas captures the human stories behind the science from a 2022 paper titled “Frequent spindle errors require structural rearrangement to complete meiosis in Zea mays” which was published by her group in 2022 in the International Journal of Molecular Sciences.

Science never works out the way we plan. As scientists, we ask questions, hypothesize and outline our goals … then reality of science occurs. The reality of science is often full of failed controls, endless troubleshooting, and sometimes strange findings that lead us in new and unpredictable directions. Our publications give the impression that we planned these scientific journeys from the beginning and do not tell the human side of the process with all of its twists and turns, dead-ends and U-turns. I want to tell you the real story behind my first publication as a faculty member with my own lab. It did not go as planned due to the COVID-19 pandemic. My lab was shut down in the middle of our investigation, and my students and I were unable to generate new data. In the beginning, it seemed like we were stranded with only control data and no story to tell, but the time away from the lab allowed us to spend more time looking carefully at wild-type cells. What seemed like a dead-end suddenly became its own story when we found something unexpected hiding within microscopy movies. Our wild-type cells were making mistakes, attempting fixes and changing directions, just like we do as scientists.

My scientific journey began with flickering green lights and a microscope (you can read more about it here). As an undergraduate, I was mesmerized by the beauty of watching living cells shuffle fluorescently labeled proteins throughout their cytoplasm. I followed this passion for microscopy into my doctoral dissertation research at Harvard University where I investigated how yeast cells build the machinery needed to pull their chromosomes apart. This machinery is a dynamic collection of long protein tubes called microtubules and other organizing proteins that help move and shuffle microtubules. I loved watching the delicate dance of chromosomes interacting with microtubules of the spindle, and I wanted to continue studying this process in my postdoctoral studies.

During postdoctoral studies at the University of Georgia, I won a fellowship from the National Science Foundation to develop a new technique in microscopy. No one had ever watched plants building their spindles in meiosis, the specialized cell division that produces egg and sperm. Other scientists had performed beautiful microscopy studies observing how mitotic spindles function inside of plant cells, but due to the technical challenges, no one had ever observed live plant cells building spindles in meiosis. I was thrilled to take on this challenge by using version of maize that had fluorescently labeled tubulin, the protein that makes up microtubules of the spindle. With this line of maize, spindles would glow fluorescent green, allowing me to image if only I could extract the meiotic cells.

Dr. Natalie Nannas

We were so busy collecting data and prepping for our mutant studies that we never really took time to analyze the wild-type cells.

After almost a year spent dissecting maize plants, I finally managed to develop a method to isolate these tiny cells and keep them alive in a growth media long enough to image them. This new method of live imaging was going to serve as the foundation of my new lab at Hamilton College, a primarily undergraduate institution. With my students, I planned to investigate the pathways governed spindle assembly. Most animal mitotic cells have a structure called a centrosome that dictates how spindles are formed; however, female animal meiotic cells lack these structures and must use other pathways to direct spindle assembly. Plants also lack centrosomes, and I wanted to inhibit these known animal pathways in our plant live imaging system.

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As I set up my lab, my students and I collected live movies of wild-type maize cells building their spindles. I told my students and myself that these movies were not the main event, they were just the control cells so we would have a baseline comparison for our experimental conditions. We were so busy collecting data and prepping for our mutant studies that we never really took the time to analyze the wild-type cells. At the surface level, they built spindles and segregated chromosomes in a generally expected amount of time, so we focused on preparing for our upcoming experiments…. then March 2020 occurred.

The pandemic forced us to slow down and look more carefully at our wild-type data, and I am grateful for the detour.

My students headed home for spring break with a warning that there may be a delay in coming back to campus due to the spread of COVID-19. None of us were prepared for the shutdown that followed. Like many colleges and universities, our campus was closed for the remainder of the spring 2020 semester and the summer of 2020. My students and I began meeting on Zoom, trying to make a new plan for our research. The only data we had to work with were the microscopy of wild-type maize cells, so we decided to spend time digging more deeply into these movies. Originally, we had only measured the total time it took to build a spindle as it would be a baseline for comparison to our mutants. We had not looked carefully at any of the intermediate time points in the assembly process. When my students looked more closely at our movies, they discovered that wild-type cells built an incorrectly shaped spindle over 60% of the time!

We found that maize meiotic cells often built spindles with three poles instead of two, and they had to actively rearrange their spindle structure to correct this mistake. We also found that in these cells, there was a delay in meiosis as cells refused to progress until this correction had been made. This is an exciting discovery as it showed that plants are error-prone in their spindle assembly, much like human female meiotic cells. Our findings also suggested that meiotic cells were monitoring their spindle shape when determining if they should move forward in meiosis. Previous work has shown that cells monitor the attachment of chromosomes to the spindle to make this decision, but our work adds a new dimension, showing that they also monitor spindle shape. As we continued to analyze our videos, we also learned that cells corrected their spindle morphology in a predictable way. They always collapsed the two poles that were closest together, creating a single pole and resulting in a correct bipolar spindle.

The image shows the first page of the paper which can be accessed here.

My students and I had begun our scientific journey planning to breeze over wild-type cells, moving on to what we envisioned would be a more exciting story of spindle mutants. The pandemic forced us to slow down and look more carefully at our wild-type data, and I am grateful for the detour. I rediscovered my love of closely watching flickering green fluorescent lights, the dance of microtubules sliding into place or making missteps and shuffling into new arrangements. Watching life attempt a complicated process, make mistakes, and try again, is a lesson that never grows old. It reminds me that our scientific journeys are just the same, they start in one direction but are fluid and constantly changing, and hopefully, they end with a functional spindle!

Read the Published Paper

Weiss, J.D., McVey, S.L., Stinebaugh, S.E., Sullivan, C.F., Dawe, R.K., and N.J. Nannas. 2022. Frequent spindle errors require structural rearrangement to complete meiosis in Zea maysInternational Journal of Molecular Sciences, 23 (8):4293–4312.

ABOUT: Stories in Science is a special series on the Civic Science Times. The main aim is to document the first-hand accounts of the human stories behind the science being published by scientists around the world. Such stories are an important element behind the civic nature of science.

SUBMISSION: Click here to access the story guidelines and submission portal. Please note that not all stories are accepted for publication. After submission, we will let you know whether we have selected the story for the review process.

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