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The Past is the Key to the Future

Peter Puleo: “In working with them and learning about how Earth science is conducted day-by-day, I learned a lot about how to do this kind of lab work, use the scientific process effectively, and think like a scientist.”

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Peter Puleo

[su_boxbox title=”About”]Pete is a graduate student in the Earth and Planetary Sciences Department at Northwestern University. He works with Dr. Yarrow Axford to gain a better understanding of how the climate has changed over the past 15,000 years through studying changes in sediment layers over time. To reconstruct the changing climate in some regions of Greenland, Pete collects lake sediments with his team and measures the stable oxygen isotope chemistry of insect parts. These bug parts record changes in lake water that are driven by climate changes over time and are preserved in the sediment (like a time capsule). He is passionate about understanding how and why the climate has changed in the past, and how it can be used to predict the future. Outside of science, Pete loves to bake and play disc golf. For more information, follow Pete on Twitter. Image byย Simon Steinbergerย fromย Pixabay.ย The story was edited by Katelyn Comeau.[/su_boxbox]

[su_boxnote note_color=”#c8c8c8″]Key Points:

  • Your experiences can shape who you are as a scientist.
  • When you are struggling or feeling lost, do not be afraid to reach out for help. Professors and classmates want to see you succeed!
  • Mentors can shape who you are as a scientist and person. Find them as early as possible and keep them close.[/su_boxnote]

Peter Puleo

[dropcap]A[/dropcap]s a researcher of past climates on Earth, I often find myself thinking of the past as holding the key to the future. There were many periods in Earthโ€™s history that were warmer than the present. As a scientist today, I can look at these older, warmer periods in Earthโ€™s history and use them as analogs for future global warming. Although this method does not perfectly reflect the climates we experience today or will experience in the future, studying these time periods greatly improves our understanding of how and why the climate has changed in the past, which we can reference in the future. As I thought about my trajectory as a student and scientist to craft this narrative, I realized this principle of the past being the key to the future held true there too. My life experiences largely sum up who I am as a scientist now and, most likely, who I will be in the future.

As a young kid growing up in a small town in Wisconsin, I was fortunate to have open access to nature. I remember filling my pockets with dirt from the yard (my mom was not happy about thatโ€ฆ) and starting a rock collection that I still have to this day. As I grew older, my interests in the natural world drew me towards science broadly as I began taking science courses. At my small high school, there were no offerings of courses in either Earth or Environmental Sciences, and unfortunately, summer programs and opportunities to learn more about STEM fields were few and far between. Thankfully, I was able to take courses in physics, chemistry, and math, which I enjoyed, but it was not until I began my undergraduate studies in college that my passions for nature and science collided.

Although I discovered my passion for climate study early in my undergraduate studies, my transition into college was not without hardships.

One of the very first courses I took in my first year at Northwestern University was a course on climate change. It was in this semester that I learned about how the global climate is changing drastically in recent years due to the combustion of fossil fuels. Furthermore, I began to appreciate that everyone on this planet experiences negative impacts and repercussions from these sudden changes in climate, with the poorest countries unfairly facing the greatest hardships. We read the book Field Notes from a Catastrophe by Elizabeth Kolbert which described rising sea levels, temperatures, droughts, and floods, all of which drew me towards understanding the complex processes underlying global climate change. This book remains one of my favorite books to this day, and I highly recommend it to anyone interested in stories and climate change. As I worked through the semester, I quickly realized that climate study and Earth science was for me since it would allow me to take part in creating new scientific knowledge, conduct my work in nature, promote education of global climate change, and make an impact on this planet and the people in it.

Although I discovered my passion for climate study early in my undergraduate studies, my transition into college was not without hardships. I struggled greatly in my first year with introductory STEM courses taught at a rigorous pace. Subjects that once felt natural to me in high school, like math and chemistry, suddenly felt out of my reach. By the end of my first year, my grades were not where I wanted them, and my motivation was dwindling.

As a first-generation STEM student, I struggled with asking for help and developing good study habits. Fortunately, I was able to make meaningful connections with older students and reach out to professors and teaching assistants in office hours for advice and studying tips. These choices helped me develop the skills I needed to succeed as a student in STEM and pass my math and chemistry classes.

At the end of my first year, I found myself on a summer trip to Colorado and Utah as part of a geology field camp after taking a course on sedimentology and stratigraphy. In terms of field gear and experience, I had none; all I had was excitement for conducting my first real field work. After my mom and I scraped together some money for the essential items, I left for the field camp and ended up having one of the most pivotal experiences of my life. There were many memorable moments on that trip: making new friends, visiting places I had never been, seeing amazing rock formations, developing observational skills, taking field notes, and camping. It was my first glimpse into being an Earth scientist, and boy was I hooked.

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In my second year of college, I realized I wanted to get involved in more hands-on research. I reached out to Dr. Beddows โ€“ my climate change seminar professor โ€“ for advice. She shared a list of labs that were looking for undergraduate research students, and one caught my eye immediately: Dr. Yarrow Axfordโ€™s Quaternary Sediment Laboratory. The Axford labโ€™s research is focused on tracking changes in lake environments and climates over time through the collection of sediment cores. Sediment cores are essentially tubes of mud taken from the bottom of lakes that can be collected using a system of ropes, pulleys, weights, and PVC tubes off the side of a boat. We lower the tube into the water and raise and lower a weight onto it to hammer it into the sediment. Suction keeps the sediment in the tube when we pull it up from the lake bottom โ€“ like when you put your thumb over the end of a straw and it stays full of water. This work seemed like the perfect combination of field work, lab work, and relevance to society โ€“ and it turned out it was.

The combination of collecting important samples and spending time with colleagues camping, cooking, flying in helicopters, laughing, and being bitten by mosquitos made this trip unforgettable.

Soon after joining the lab, I was working with Dr. Axford, graduate students, and other undergraduates on research projects. In working with them and learning about how Earth science is conducted day-by-day, I learned a lot about how to do this kind of lab work, use the scientific process effectively, and think like a scientist. After a year and a half of working in the lab and contributing to other projects, I wanted to collect samples of my own for a more independent project. I got approval to go to Wisconsin with members of my lab group where we collected sediment cores from Geneva Lake, located on the southern border of Wisconsin. I analyzed this data and found some interesting results which I ended up writing my thesis about and later publishing.

Soon after this work, I was asked to join Dr. Axfordโ€™s team on a month-long field campaign of camping and sediment coring in southern Greenland. I will never forget that day I woke up and saw the email inviting me on the trip. I jumped up and down with joy and called my mom to share the news that I was a part of a team that was making a difference in climate research. I was so overjoyed for the opportunity and also very thankful for the department, Dr. Beddows, and Dr. Axford for dedicating time and money to get adequate equipment and training for me.

The combination of collecting important samples and spending time with colleagues camping, cooking, flying in helicopters, laughing, and being bitten by mosquitos made this trip unforgettable. From the samples collected on this trip, we generated data that informed us of how mountain glaciers and temperatures changed over the past 10,000 years in the region. Besides the sense of accomplishment and fun of conducting interesting research, I made four life-long friends and colleagues on that trip.

In my last year of college, I decided to apply to graduate schools. My experiences with research had been incredibly rewarding and I wanted to continue conducting research in the field of Earth Science., During a discussion with Professor Axford late in the application process, I realized that staying at Northwestern for my graduate studies was an option for me. I not only loved how the research I was conducting contributed to our understanding of how climates changed in the past and how they might change in the future, but also working beside my current colleagues and mentors. It felt like home in the Axford lab. So, I applied to Northwestern Universityโ€™s Earth and Planetary Sciences graduate program. I, was accepted and chose to stay here and continue my work on climate studies. I am currently reconstructing past climate using the stable oxygen isotope chemistry of bug parts found in sediment cores I collected from Greenland with my colleagues. This chemical ratio is driven by changes in climate and precipitation, making it an extremely useful chemical proxy for paleoclimate reconstruction. I am excited to return for more samples in the future and to continue sharing my knowledge of climate change with others.

Each of the experiences I shared here exceedingly influenced my career trajectory and make up who I am as a scientist today. I would argue that my past has been the key to my present and future. Each experience led to the next, but not always easily or straightforwardly.

One common theme through each of these experiences was unwavering support from those around me. My mentors. My professors. My lab mates. Without their support and guidance, I would not be where I am today. And for that, I will forever be grateful. I cannot wait to be able to be a mentor for students in the future because I know how large of an impact mentorship and guidance can make as a recipient of great guidance in my past.

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CivicSciTimes - Stories in Science

Unexpected Stories and Spindle Mistakes: Discovering that Wild-type Cells are Full of Surprises

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Natalie Nannas

Natalie Nannas is an Associate Professor of Biology at Hamilton College in Clinton, NY. She teaches courses in genetics, molecular biology, and bioethics. Dr. Nannas graduated from Grinnell College with bachelor’s degrees in biological chemistry and French. She received her Masterโ€™s and PhD from Harvard University in molecular biology and genetics. Dr. Nannas conducted her postdoctoral research at the University of Georgia where she won a National Science Foundation Plant Genome Postdoctoral Fellowship. At Hamilton College, Dr. Nannas enjoys teaching and sharing her passion for microscopy with her undergraduate research students. When not glued to a microscope, she loves spending time with her husband and two daughters. The narrative below by Natalie Nannas captures the human stories behind the science from a 2022 paper titled โ€œFrequent spindle errors require structural rearrangement to complete meiosis in Zea maysโ€ which was published by her group in 2022 in the International Journal of Molecular Sciences.

Science never works out the way we plan. As scientists, we ask questions, hypothesize and outline our goals โ€ฆ then reality of science occurs. The reality of science is often full of failed controls, endless troubleshooting, and sometimes strange findings that lead us in new and unpredictable directions. Our publications give the impression that we planned these scientific journeys from the beginning and do not tell the human side of the process with all of its twists and turns, dead-ends and U-turns. I want to tell you the real story behind my first publication as a faculty member with my own lab. It did not go as planned due to the COVID-19 pandemic. My lab was shut down in the middle of our investigation, and my students and I were unable to generate new data. In the beginning, it seemed like we were stranded with only control data and no story to tell, but the time away from the lab allowed us to spend more time looking carefully at wild-type cells. What seemed like a dead-end suddenly became its own story when we found something unexpected hiding within microscopy movies. Our wild-type cells were making mistakes, attempting fixes and changing directions, just like we do as scientists.

My scientific journey began with flickering green lights and a microscope (you can read more about it here). As an undergraduate, I was mesmerized by the beauty of watching living cells shuffle fluorescently labeled proteins throughout their cytoplasm. I followed this passion for microscopy into my doctoral dissertation research at Harvard University where I investigated how yeast cells build the machinery needed to pull their chromosomes apart. This machinery is a dynamic collection of long protein tubes called microtubules and other organizing proteins that help move and shuffle microtubules. I loved watching the delicate dance of chromosomes interacting with microtubules of the spindle, and I wanted to continue studying this process in my postdoctoral studies.

During postdoctoral studies at the University of Georgia, I won a fellowship from the National Science Foundation to develop a new technique in microscopy. No one had ever watched plants building their spindles in meiosis, the specialized cell division that produces egg and sperm. Other scientists had performed beautiful microscopy studies observing how mitotic spindles function inside of plant cells, but due to the technical challenges, no one had ever observed live plant cells building spindles in meiosis. I was thrilled to take on this challenge by using version of maize that had fluorescently labeled tubulin, the protein that makes up microtubules of the spindle. With this line of maize, spindles would glow fluorescent green, allowing me to image if only I could extract the meiotic cells.

Dr. Natalie Nannas

We were so busy collecting data and prepping for our mutant studies that we never really took time to analyze the wild-type cells.

After almost a year spent dissecting maize plants, I finally managed to develop a method to isolate these tiny cells and keep them alive in a growth media long enough to image them. This new method of live imaging was going to serve as the foundation of my new lab at Hamilton College, a primarily undergraduate institution. With my students, I planned to investigate the pathways governed spindle assembly. Most animal mitotic cells have a structure called a centrosome that dictates how spindles are formed; however, female animal meiotic cells lack these structures and must use other pathways to direct spindle assembly. Plants also lack centrosomes, and I wanted to inhibit these known animal pathways in our plant live imaging system.

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As I set up my lab, my students and I collected live movies of wild-type maize cells building their spindles. I told my students and myself that these movies were not the main event, they were just the control cells so we would have a baseline comparison for our experimental conditions. We were so busy collecting data and prepping for our mutant studies that we never really took the time to analyze the wild-type cells. At the surface level, they built spindles and segregated chromosomes in a generally expected amount of time, so we focused on preparing for our upcoming experimentsโ€ฆ. then March 2020 occurred.

The pandemic forced us to slow down and look more carefully at our wild-type data, and I am grateful for the detour.

My students headed home for spring break with a warning that there may be a delay in coming back to campus due to the spread of COVID-19. None of us were prepared for the shutdown that followed. Like many colleges and universities, our campus was closed for the remainder of the spring 2020 semester and the summer of 2020. My students and I began meeting on Zoom, trying to make a new plan for our research. The only data we had to work with were the microscopy of wild-type maize cells, so we decided to spend time digging more deeply into these movies. Originally, we had only measured the total time it took to build a spindle as it would be a baseline for comparison to our mutants. We had not looked carefully at any of the intermediate time points in the assembly process. When my students looked more closely at our movies, they discovered that wild-type cells built an incorrectly shaped spindle over 60% of the time!

We found that maize meiotic cells often built spindles with three poles instead of two, and they had to actively rearrange their spindle structure to correct this mistake. We also found that in these cells, there was a delay in meiosis as cells refused to progress until this correction had been made. This is an exciting discovery as it showed that plants are error-prone in their spindle assembly, much like human female meiotic cells. Our findings also suggested that meiotic cells were monitoring their spindle shape when determining if they should move forward in meiosis. Previous work has shown that cells monitor the attachment of chromosomes to the spindle to make this decision, but our work adds a new dimension, showing that they also monitor spindle shape. As we continued to analyze our videos, we also learned that cells corrected their spindle morphology in a predictable way. They always collapsed the two poles that were closest together, creating a single pole and resulting in a correct bipolar spindle.

The image shows the first page of the paper which can be accessed here.

My students and I had begun our scientific journey planning to breeze over wild-type cells, moving on to what we envisioned would be a more exciting story of spindle mutants. The pandemic forced us to slow down and look more carefully at our wild-type data, and I am grateful for the detour. I rediscovered my love of closely watching flickering green fluorescent lights, the dance of microtubules sliding into place or making missteps and shuffling into new arrangements. Watching life attempt a complicated process, make mistakes, and try again, is a lesson that never grows old. It reminds me that our scientific journeys are just the same, they start in one direction but are fluid and constantly changing, and hopefully, they end with a functional spindle!

Read the Published Paper

Weiss, J.D., McVey, S.L., Stinebaugh, S.E., Sullivan, C.F., Dawe, R.K., and N.J. Nannas. 2022. Frequent spindle errors require structural rearrangement to complete meiosis in Zea maysInternational Journal of Molecular Sciences, 23 (8):4293โ€“4312.

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ABOUT: Stories in Science is a special series on the Civic Science Times. The main aim is to document the first-hand accounts of the human stories behind the science being published by scientists around the world. Such stories are an important element behind the civic nature of science.

SUBMISSION: Click here to access the story guidelines and submission portal. Please note that not all stories are accepted for publication. After submission, we will let you know whether we have selected the story for the review process.

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