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My experience as a Kurdish Undergraduate Scientist in Iraq

Soma Sardar Barawi: “As a Kurdish nationalist first, and a future forensic biologist second, I desperately want to serve my homeland through the use of modern forensic technology.”

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Soma Sardar Barawi

[su_boxbox title=”About”]Soma Sardar Barawi is an undergraduate scientific researcher currently in her final senior year studying General Biology at the Department of Biology/ University of Sulaimani in Kurdistan, Iraq. She loves reading and petting cats! You can email her at: [email protected].[/su_boxbox]

[su_boxnote note_color=”#c8c8c8″]Story Key Points:

  • Follow and trust your curiosity. It can completely transform your situation and mindset.
  • Donโ€™t limit yourself to thinking about one field or profession. Explore and find new connections between different fields.
  • If you are a student, immerse yourself in the glorious realm of undergraduate research when possible. You never know what you may discover along the way.[/su_boxnote]

[dropcap]M[/dropcap]y undergraduate education was completely transformed during the summer of 2017 after my freshman year in college. I mean, I excelled in my classes and had good grades overall. But something was simultaneously missing at the same time. I was bored of sitting remotely in class, quietly taking notes, and nonchalantly completing my exams at the end of each year. I simply craved more. As a future scientist, I wanted to actually apply first-hand what I was learning from these classes. I felt like I had zero background in the practical field, and I was curious about how to safely handle laboratory equipment and perform experiments on my own. In other words, I was curious about the enormous and glorious realm of scientific research.

Soma Sardar Barawi

But the question, was how? How was I supposed to get access to these things? Opportunities seldom arise for curious and intellectual students living in Iraq, a country prone to challenging environments, and not to mention high-profile war zone and brutal economic crises. I wanted to test my abilities outside of the classroom, and I was specifically fascinated about the world of tiny microorganisms involved in every aspect of human life. I desperately wanted to study bacterial genetics and their complicated roles in human disease. Furthermore, understanding their genomic sequences and their implications in pathogenesis intrigued me to the bone. I know it might seem clichรฉ, but additionally, yes, I was yet another child who grew up watching all of the forensics/ crime scene investigation (CSI) movies and series. If only I could have expanded my limited knowledge in microbial genetics as the first step, I could then pursue my life-long dream of becoming a forensic microbiologist. However, forensics as a field in Iraq has yet to be applied on a popular, large-scale.

As an American/Kurdish citizen living in Kurdistan, I found it crucial for myself to follow these aspirations to accumulate a strong background in this specific, and relevant research field. You see, as Kurds living in the Kurdistan region of Iraq, we have a long history of thirsting for our independence. After the fall of the Ottoman Empire, the Kurds were amiably promised a state of their own. Nevertheless, this was over a century ago. Kurdistan, basically meaning land of the Kurds, was torn apart and finds its citizens scattered between 4 surrounding countries: Iraq, Syria, Turkey, and Iran. The Kurds in Iraq, as being culturally and linguistically different from the Arab majority in the country, have long suffered persecution and oppression under Iraqi rule. On March 16, 1988, the largest and most detrimental chemical weapons (mustard gas) attack was executed on the Kurdish people in the Kurdish town of Halabja by the notorious Iraqi dictator, Saddam Hussein and his party. Similar attacks were seen in nearby Kurdish cities. The effects of this horrendous genocide and ethnic cleansing are still seen in the town today, as hundreds of babies are still being born with severe birth defects, mutations, genetic diseases and complications.

As a freshman, these ideas haunted me. I decided to search in vain for a teacher/ supervisor who could point me in the right direction. Unfortunately, teachers rarely welcomed students into their labs with open arms.

The Halabja Massacre is known as Bloody Friday, where Kurds gather in a state of despair to mourn for the hundreds of thousands of Kurds who were brutally massacred in the systematic series of events known as the Al-Anfal Genocide, or Kurdish Genocide taking place between the years 1986 through 1989. This event was aimed at eliminating Kurdish identity, nationalism and resistance. All over Iraq, Iraqi guards would randomly round up Kurdish men, women, and children, and dispose of them in mass-grave sites dug up for their bodies. Each adult would be shot nine times, and each child, four. Whoever wasnโ€™t shot would be buried alive. This way, ninety percent of Kurdish towns were subjected to mass murder and torture, chemical warfare and gas attacks, mass deportation, and mass disappearances of hundreds of thousands of Kurdish citizens that simply vanished without a trace.

However, I learned that in 2005, forensic archaeologists and anthropologists started exhuming and analyzing the bodies of the victims to uncover major evidence of the Kurdish genocide. This would finally lead to the conviction of Saddam Hussein and his allies for their barbaric crimes against humanity. To this day, Kurdistan remains an interesting plethora of opportunities for the emergence of forensics. This was the epitome of my inspiration. As a Kurdish nationalist first, and a future forensic biologist second, I desperately want to serve my homeland through the use of modern forensic technology. I had never heard of a forensic scientist/ biologist in my city, and as silly as it sounded, I kind of hoped I would be the first.

As a freshman, these ideas haunted me. I decided to search in vain for a teacher/ supervisor who could point me in the right direction. Unfortunately, teachers rarely welcomed students into their labs with open arms. Materials and supplies were very expensive, and thus, too valuable to be wasted on students too curious for their own good. However, lucky enough, I got into contact with an extremely intelligent assistant professor with a M.S. in Biotechnology and Genetic Engineering, and a Ph.D. in Microbial Biotechnology. He was also in charge of a Microbiology Research Lab at my university. This led us to the idea of starting a Microbiology Training Program. It was an absolute dream come true; he instantly agreed to train myself and a few of my friends. The main objective of the program was to assist curious and creative students to understand the complete basics of General Microbiology and how to actually apply those concepts in real-life experiments. Later on, students would be encouraged to formulate their own ideas and hypotheses, and thus be introduced to the enormous world of research.

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Following my training as a freshman in 2017, I first started research on antibiotics. I had just started my junior year at the time, and a few classes on antibiotic resistance immediately caught my attention. I first started work on extracting natural antimicrobial products limited to the Kurdistan region. But when my own results werenโ€™t deemed promising, my colleagues and I joined forces to conduct a larger project. Throughout the course of a year and a half, we conducted research and discovered a strong antimicrobial plant extract as an alternative source for antibiotics.

Antibiotic resistance is an extremely huge public health threat that occurs due to the inappropriate use of antibiotics, and the evolutionary resistance mechanisms adapted by microorganisms, including their ability to form biofilms, or transfer virulence and resistance genes through plasmids. Plasmids are extra-chromosomal DNA elements in bacteria that have the ability to self-replicate. They also contain antibiotic resistance genes which can be spread easily from one bacterium to another. This has caused antibiotic resistance to spread at an alarming rate, rendering antibiotics ineffective and causing many complications and deaths worldwide.

After completing our project, we coincidently discovered an open call for submission (with 3 days remaining) for the 2nd World Congress for Undergraduate Research in Oldenburg, Germany. We applied eagerly. After a few months, we were extremely excited to know that we were the first group of high scorers and the only group from Iraq and the surrounding countries accepted to the conference. Iraq ranked 7th on the list of countries who applied! Rarely do these miracles happen for students like us living in such challenging countries. Thankfully, our university generously agreed to completely support us financially, as this was deemed to be a very huge and successful event for us as students, our university, and for Kurdistan, Iraq.

The World Congress for Undergraduate Research is an extremely prestigious congress where highly active students worldwide are invited to share their research, participate in discussions about global issues, and create international research collaborations. We successfully presented our research there in May 2019, and were introduced to many other young scientists, researchers, and ideas! Benefits of undergraduate research includes developing numerous practical and independent critical thinking skills, as well as oral and written communication skills. The possibilities of acquiring scholarship opportunities and getting accepted in graduate programs abroad is also increased significantly.

Becoming an independent researcher was the highlight of my undergraduate education.

Our central goal was to let students living in Kurdistan know that they are capable of doing great things. We were the first group of undergraduate students at the University of Sulaimani to conduct such research and actually present it at a large international conference. Undergraduate research is the epitome of gaining a hands-on experience thatโ€™s very beneficial for future academic careers. If more students realize this and are motivated to do research, they then have the potential to present their work at international meetings like the World Congress. Despite living under such hard circumstances, we wanted other countries and other universities to know that Kurdistan was alive, and that we were capable of doing just as much amazing things as they were.

Becoming an independent researcher was the highlight of my undergraduate education. I have developed a strong research foundation that has opened many doors and broadened many career options for me. I have expanded my knowledge in several research fields, and Iโ€™ve discovered my calling to be a scientist through my experiments. Fast forward to three years later and here I am: a senior completing my last semester in General Biology. My last project as an undergraduate focused on the promising fields of Nanotechnology and Microbial Biotechnology, coming together in a ground-breaking interconnection to exploit microorganisms as bio-factories for synthesizing nanoparticles. These nanoparticles have found their potential roles in extremely important applications such as in gene and drug-delivery systems in medicine and bioremediating agents in polluted environments. Amazingly, my project has been accepted yet again to another annual conference, Excellence in Undergraduate Research, Entrepreneurship, and Creative Achievement (EURECA) in Egypt (April 2020).

Iโ€™m extremely humbled by the opportunities I have been introduced to. If it wasnโ€™t for my own curiosity as a freshman, I guess I never wouldโ€™ve entered the world of research. Iโ€™ve broadened my goals and aspirations, and I donโ€™t want to be limited to one specific research field forever. My passion and the love I have for science have deepened incredibly and shaped me into the woman and researcher I am today. And honestly, Iโ€™m quite excited for what the future holds! Who knows? Maybe Iโ€™ll find myself working at the intersection of diverse research fields. Stay tuned.

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CivicSciTimes - Stories in Science

Unexpected Stories and Spindle Mistakes: Discovering that Wild-type Cells are Full of Surprises

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Natalie Nannas

Natalie Nannas is an Associate Professor of Biology at Hamilton College in Clinton, NY. She teaches courses in genetics, molecular biology, and bioethics. Dr. Nannas graduated from Grinnell College with bachelor’s degrees in biological chemistry and French. She received her Masterโ€™s and PhD from Harvard University in molecular biology and genetics. Dr. Nannas conducted her postdoctoral research at the University of Georgia where she won a National Science Foundation Plant Genome Postdoctoral Fellowship. At Hamilton College, Dr. Nannas enjoys teaching and sharing her passion for microscopy with her undergraduate research students. When not glued to a microscope, she loves spending time with her husband and two daughters. The narrative below by Natalie Nannas captures the human stories behind the science from a 2022 paper titled โ€œFrequent spindle errors require structural rearrangement to complete meiosis in Zea maysโ€ which was published by her group in 2022 in the International Journal of Molecular Sciences.

Science never works out the way we plan. As scientists, we ask questions, hypothesize and outline our goals โ€ฆ then reality of science occurs. The reality of science is often full of failed controls, endless troubleshooting, and sometimes strange findings that lead us in new and unpredictable directions. Our publications give the impression that we planned these scientific journeys from the beginning and do not tell the human side of the process with all of its twists and turns, dead-ends and U-turns. I want to tell you the real story behind my first publication as a faculty member with my own lab. It did not go as planned due to the COVID-19 pandemic. My lab was shut down in the middle of our investigation, and my students and I were unable to generate new data. In the beginning, it seemed like we were stranded with only control data and no story to tell, but the time away from the lab allowed us to spend more time looking carefully at wild-type cells. What seemed like a dead-end suddenly became its own story when we found something unexpected hiding within microscopy movies. Our wild-type cells were making mistakes, attempting fixes and changing directions, just like we do as scientists.

My scientific journey began with flickering green lights and a microscope (you can read more about it here). As an undergraduate, I was mesmerized by the beauty of watching living cells shuffle fluorescently labeled proteins throughout their cytoplasm. I followed this passion for microscopy into my doctoral dissertation research at Harvard University where I investigated how yeast cells build the machinery needed to pull their chromosomes apart. This machinery is a dynamic collection of long protein tubes called microtubules and other organizing proteins that help move and shuffle microtubules. I loved watching the delicate dance of chromosomes interacting with microtubules of the spindle, and I wanted to continue studying this process in my postdoctoral studies.

During postdoctoral studies at the University of Georgia, I won a fellowship from the National Science Foundation to develop a new technique in microscopy. No one had ever watched plants building their spindles in meiosis, the specialized cell division that produces egg and sperm. Other scientists had performed beautiful microscopy studies observing how mitotic spindles function inside of plant cells, but due to the technical challenges, no one had ever observed live plant cells building spindles in meiosis. I was thrilled to take on this challenge by using version of maize that had fluorescently labeled tubulin, the protein that makes up microtubules of the spindle. With this line of maize, spindles would glow fluorescent green, allowing me to image if only I could extract the meiotic cells.

Dr. Natalie Nannas

We were so busy collecting data and prepping for our mutant studies that we never really took time to analyze the wild-type cells.

After almost a year spent dissecting maize plants, I finally managed to develop a method to isolate these tiny cells and keep them alive in a growth media long enough to image them. This new method of live imaging was going to serve as the foundation of my new lab at Hamilton College, a primarily undergraduate institution. With my students, I planned to investigate the pathways governed spindle assembly. Most animal mitotic cells have a structure called a centrosome that dictates how spindles are formed; however, female animal meiotic cells lack these structures and must use other pathways to direct spindle assembly. Plants also lack centrosomes, and I wanted to inhibit these known animal pathways in our plant live imaging system.

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As I set up my lab, my students and I collected live movies of wild-type maize cells building their spindles. I told my students and myself that these movies were not the main event, they were just the control cells so we would have a baseline comparison for our experimental conditions. We were so busy collecting data and prepping for our mutant studies that we never really took the time to analyze the wild-type cells. At the surface level, they built spindles and segregated chromosomes in a generally expected amount of time, so we focused on preparing for our upcoming experimentsโ€ฆ. then March 2020 occurred.

The pandemic forced us to slow down and look more carefully at our wild-type data, and I am grateful for the detour.

My students headed home for spring break with a warning that there may be a delay in coming back to campus due to the spread of COVID-19. None of us were prepared for the shutdown that followed. Like many colleges and universities, our campus was closed for the remainder of the spring 2020 semester and the summer of 2020. My students and I began meeting on Zoom, trying to make a new plan for our research. The only data we had to work with were the microscopy of wild-type maize cells, so we decided to spend time digging more deeply into these movies. Originally, we had only measured the total time it took to build a spindle as it would be a baseline for comparison to our mutants. We had not looked carefully at any of the intermediate time points in the assembly process. When my students looked more closely at our movies, they discovered that wild-type cells built an incorrectly shaped spindle over 60% of the time!

We found that maize meiotic cells often built spindles with three poles instead of two, and they had to actively rearrange their spindle structure to correct this mistake. We also found that in these cells, there was a delay in meiosis as cells refused to progress until this correction had been made. This is an exciting discovery as it showed that plants are error-prone in their spindle assembly, much like human female meiotic cells. Our findings also suggested that meiotic cells were monitoring their spindle shape when determining if they should move forward in meiosis. Previous work has shown that cells monitor the attachment of chromosomes to the spindle to make this decision, but our work adds a new dimension, showing that they also monitor spindle shape. As we continued to analyze our videos, we also learned that cells corrected their spindle morphology in a predictable way. They always collapsed the two poles that were closest together, creating a single pole and resulting in a correct bipolar spindle.

The image shows the first page of the paper which can be accessed here.

My students and I had begun our scientific journey planning to breeze over wild-type cells, moving on to what we envisioned would be a more exciting story of spindle mutants. The pandemic forced us to slow down and look more carefully at our wild-type data, and I am grateful for the detour. I rediscovered my love of closely watching flickering green fluorescent lights, the dance of microtubules sliding into place or making missteps and shuffling into new arrangements. Watching life attempt a complicated process, make mistakes, and try again, is a lesson that never grows old. It reminds me that our scientific journeys are just the same, they start in one direction but are fluid and constantly changing, and hopefully, they end with a functional spindle!

Read the Published Paper

Weiss, J.D., McVey, S.L., Stinebaugh, S.E., Sullivan, C.F., Dawe, R.K., and N.J. Nannas. 2022. Frequent spindle errors require structural rearrangement to complete meiosis in Zea maysInternational Journal of Molecular Sciences, 23 (8):4293โ€“4312.

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ABOUT: Stories in Science is a special series on the Civic Science Times. The main aim is to document the first-hand accounts of the human stories behind the science being published by scientists around the world. Such stories are an important element behind the civic nature of science.

SUBMISSION: Click here to access the story guidelines and submission portal. Please note that not all stories are accepted for publication. After submission, we will let you know whether we have selected the story for the review process.

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